| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 40.6 kDa with a Cys on N-terminus, a single non-glycosylated polypeptide chain containing 366 amino acids. But it migrates with an apparent molecular mass of 46 kDa in SDS-PAGE. |
| AA Sequence: |
CKEETPETPE TDSEEEVTIK ANLIFANGST QTAEFKGTFE KATSEAYAYA DTLKKDNGEY TVDVADKGYT LNIKFAGKEK TPEEPKEEVT IKANLIYADG KTQTAEFKGT FEEATAEAYR YADALKKDNG EYTVDVADKG YTLNIKFAGK EKTPEEPKEE VTIKANLIYA DGKTQTAEFK GTFEEATAEA YRYADLLAKE NGKYTVDVAD KGYTLNIKFA GKEKTPEEPK EEVTIKANLI YADGKTQTAE FKGTFAEATA EAYRYADLLA KENGKYTADL EDGGYTINIR FAGKKVDEKP EEKEQVTIKE NIYFEDGTVQ TATFKGTFAE ATAEAYRYAD LLSKEHGKYT ADLEDGGYTI NIRFAG |
| Purity: |
> 95% by SDS-PAGE analysis. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized with no additive. |
| Reconstitution: |
Dissolve in distilled water or saline. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Recombinant Protein L contains 5 kappa-binding domain. Protein L, with its five kappa-binding domains, possesses a distinctive capability to engage with kappa light chains without disrupting the antigen-binding site of antibodies. This unique feature enables Protein L to interact with a broader spectrum of Ig classes and subclasses compared to alternative antibody-binding proteins. |
| Concentration for 1 unity OD at 280 nm: |
1.132 mg/mL |
| Specificity: |
The recombinant Protein L is a genetically engineered protein containing 5 IgG-binding regions of protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein L to ensure the maximum specific IgG binding. The recombinant Protein L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein L binds to human, mouse, rat and pig IgG. |
| Endotoxin Level: |
Less than 0.1 EU/μg of Cys-Protein L as determined by LAL method. |
