High-Throughput Sequencing
High-throughput sequencing technology, also known as next-generation sequencing (NGS), is a technology that can sequence hundreds of thousands to millions of nucleic acid molecules at a time. Compared to traditional first-generation sequencing methods, such as Sanger sequencing, high-throughput sequencing offers unparalleled speed and accuracy, making it an essential tool for researchers in a wide range of fields, including oral disease research.
At Alta Stomatology, we offer a comprehensive range of high-throughput sequencing services to meet the diverse needs of our customers. Our one-stop solutions include sample preparation, library construction, high-throughput sequencing, bioinformatics routine analysis, and customized analysis. Whether you are looking to sequence a whole genome, transcriptome, small RNA, PCR amplification, or amplicon sequences, we can deliver high-quality results quickly and efficiently. If you have any needs, please feel free to contact us.
Our Services
Genome Sequencing
Genome sequencing provides a complete picture of the genetic material within an organism. At Alta Stomatology, we utilize high-throughput sequencing to perform whole-genome sequencing, which is crucial for identifying genetic variations associated with oral diseases. Our services include:
| Whole Genome Sequencing (WGS) |
| This service involves sequencing the entire genome of an organism, providing insights into genetic mutations, structural variations, and evolutionary relationships. WGS is essential for understanding the genetic basis of oral diseases, identifying potential biomarkers, and developing personalized treatment strategies. |
| Targeted Genome Sequencing |
| We offer targeted sequencing of specific genomic regions of interest. This approach is useful for investigating particular genes or loci known to be associated with oral health and disease. |
Transcriptome Sequencing
Transcriptome sequencing, or RNA-seq, allows for the comprehensive analysis of gene expression. By sequencing RNA transcripts, researchers can understand how genes are regulated and expressed in different conditions. Our transcriptome sequencing services include:
| mRNA Sequencing |
| This service focuses on sequencing messenger RNA (mRNA) molecules to study gene expression profiles. mRNA sequencing helps in identifying differentially expressed genes, splicing variants, and novel transcripts involved in oral diseases. |
| Total RNA Sequencing |
| This includes sequencing all RNA molecules, including mRNA, ribosomal RNA (rRNA), transfer RNA (tRNA), and non-coding RNAs (ncRNAs). Total RNA sequencing provides a holistic view of the transcriptome, capturing regulatory RNAs that play critical roles in gene regulation and oral disease processes. |
sRNA Sequencing
Small RNA (sRNA) molecules, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), play crucial roles in gene regulation. Our sRNA sequencing services enable the study of these regulatory RNAs, which are often implicated in oral diseases. Our services include:
| miRNA Sequencing |
| Identifying and quantifying miRNAs to understand their role in gene regulation and oral disease pathways. MiRNAs are known to regulate a wide array of biological processes, including inflammation, immune response, and cellular differentiation. |
| siRNA Sequencing |
| Investigating the role of siRNAs in gene silencing and their potential therapeutic applications in oral diseases. siRNA sequencing can help identify targets for gene knockdown experiments and RNA-based therapies. |
PCR Amplification Sequencing
Polymerase Chain Reaction (PCR) amplification is a widely used technique to amplify specific DNA fragments. Combining PCR with high-throughput sequencing allows for the detailed analysis of genetic regions of interest. Our PCR amplification sequencing services include:
| Targeted PCR Sequencing |
| Amplifying and sequencing specific genomic regions to investigate genetic variations associated with oral diseases. This approach is useful for validating candidate genes and confirming mutations identified through other sequencing methods. |
| Long-range PCR Sequencing |
| Amplifying and sequencing longer DNA fragments to study structural variations and complex genomic regions. Long-range PCR sequencing is beneficial for analyzing repetitive regions, large deletions, and insertions. |
16S/18S/ITS Amplicon Sequencing
Microbial communities play a significant role in oral health and disease. Amplicon sequencing of ribosomal RNA (rRNA) genes, such as 16S (bacteria), 18S (eukaryotes), and Internal Transcribed Spacer (ITS) regions, enables the characterization of microbial diversity. Our services include:
| 16S rRNA Gene Sequencing |
| Profiling bacterial communities within oral samples to understand the role of microbiota in oral health and disease. This service helps identify pathogenic bacteria, commensal microorganisms, and microbial dysbiosis associated with conditions such as periodontitis and dental caries. |
| 18S rRNA Gene Sequencing |
| Analyzing eukaryotic microbial communities, including fungi and protozoa, within oral samples. This service is essential for studying the role of eukaryotic microbes in oral infections and their interactions with bacterial communities. |
| ITS Sequencing |
| Targeting the ITS regions to profile fungal communities within oral samples. ITS sequencing is particularly useful for identifying fungal species and understanding their role in oral health and disease. |
Why Choose Alta Stomatology?
- Experience Team and Advanced Technology
Our team comprises highly skilled scientists and technicians with extensive experience in sequencing technologies and oral health research. In addition, we utilize the latest high-throughput sequencing platforms and bioinformatics tools to support our sequencing services, ensuring that we deliver accurate, high-quality data.
We offer a complete suite of services that cover every step of the high-throughput sequencing process. From sample preparation and library construction to sequencing and bioinformatics analysis, our integrated approach ensures seamless workflow and high-quality outcomes. Our services are designed to meet the diverse needs of our clients, whether they require routine analysis or customized research solutions.
Quality is at the core of our services. Alta Stomatology adheres to stringent quality control measures at every stage of the sequencing process. From sample integrity checks to rigorous data validation, we guarantee the highest standards of accuracy and reliability.
For research use only. Not
intended for any clinical use.
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