| Target: |
IL-6 (Interleukin 6) |
| Binding Specificity: |
AA 25-211 |
| Reactivity: |
Mouse |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This IL-6 antibody is un-conjugated |
| Application: |
ELISA, western blotting (WB) |
| Purpose: |
Rabbit IgG polyclonal antibody for Interleukin-6(IL6) detection. Tested with WB, ELISA in Mouse. |
| Characteristics: |
Rabbit IgG polyclonal antibody for interleukin 6 (interferon, beta 2)(IL6) detection. Tested with WB in mouse.
Gene name: interleukin 6 (interferon, beta 2).
Protein name: interleukin-6. |
| Purification: |
Immunogen affinity purified. |
| Immunogen: |
E. coli-derived mouse IL-6 recombinant protein (Position: F25-T211). Mouse IL-6 shares 40% and 85% amino acid (aa) sequences identity with human and rat IL-6, respectively. |
| Isotype: |
IgG |
| Format: |
Lyophilized |
| Concentration: |
500 μg/mL |
| Reconstitution: |
Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL. |
| Buffer: |
Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg sodium azide. |
| Precaution of Use: |
This product contains sodium azide: a poisonous and hazardous substance which should be handled by trained staff only. |
| Handling Advice: |
Avoid repeated freezing and thawing. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
At - 20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at - 20°C for a longer time. |
| Alternative Name: |
IL6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
The IL6 gene in humans encodes Interleukin-6 (IL-6), a protein with dual roles as both a pro-inflammatory and anti-inflammatory cytokine. T cells and macrophages secrete IL-6 to trigger immune responses, particularly in cases of trauma like burns or tissue damage that leads to inflammation. IL-6 plays a crucial role in inducing fever and the acute phase response. Additionally, IL-6 is vital for the growth of hybridomas and is commonly included in cloning media such as briclone. Bowcock et al. (1988) identified the IL6 gene on chromosome 7p21, while Sutherland et al. (1988) mapped it to chromosome 7p15 using in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines. |
