| Target: |
IL-6 (Interleukin 6) |
| Binding Specificity: |
AA 25-211 |
| Reactivity: |
Rat |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This IL-6 antibody is un-conjugated |
| Application: |
Western blotting (WB) |
| Purpose: |
Rabbit IgG polyclonal antibody for interleukin 6 (interferon, beta 2)(IL6) detection. Tested with WB in Rat. |
| Characteristics: |
Rabbit IgG polyclonal antibody for interleukin 6 (interferon, beta 2)(IL6) detection. Tested with WB in rat.
Gene name: interleukin 6 (interferon, beta 2).
Protein name: interleukin-6. |
| Purification: |
Immunogen affinity purified. |
| Immunogen: |
E. coli-derived rat IL-6 recombinant protein (position: F25-T211). Rat IL-6 shares 86% amino acid (aa) sequence identity with mouse IL-6. |
| Isotype: |
IgG |
| Format: |
Lyophilized |
| Concentration: |
500 μg/mL |
| Reconstitution: |
Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL. |
| Buffer: |
Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg sodium azide. |
| Precaution of Use: |
This product contains sodium azide: a poisonous and hazardous substance which should be handled by trained staff only. |
| Handling Advice: |
Avoid repeated freezing and thawing. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
At - 20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at - 20°C for a longer time. |
| Alternative Name: |
IL6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
The IL6 gene in humans encodes the protein Interleukin-6 (IL-6), which functions as both a pro-inflammatory and anti-inflammatory cytokine. It is produced by T cells and macrophages to prompt the immune response, particularly in cases of trauma like burns or tissue damage leading to inflammation. IL-6 plays a crucial role in inducing fever and the acute phase response. Moreover, it is vital for the growth of hybridomas and is commonly included in cloning media like briclone. Bowcock et al. (1988) positioned the IL6 gene on chromosome 7p21. Through techniques such as in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) further pinpointed the IL-6 gene to chromosome 7p15. |
