| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Human |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This IL-6 antibody is un-conjugated |
| Application: |
Enzyme immunoassay (EIA), functional studies (Func), immunofluorescence (IF), immunohistochemistry (paraffin-embedded sections) (IHC (p)), western blotting (WB) |
| Purification: |
Affinity chromatography employing immobilized hIL-6 matrix. |
| Immunogen: |
Highly pure (>98%) E.coli derived recombinant human interleukin 6. |
| Format: |
Liquid |
| Reconstitution: |
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL. |
| Buffer: |
PBS, pH 7.2 without preservatives. |
| Handling Advice: |
Avoid repeated freezing and thawing. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Prior to reconstitution store at 2 - 8°C. Following reconstitution store undiluted at 2 - 8°C for one month or (in aliquots) at - 20°C for longer. |
| Alternative Name: |
Interleukin-6 (IL6) |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
Interleukin 6 (IL6) is a versatile 24 kDa protein initially identified in the medium of RNA-stimulated fibroblastoid cells. Its expression is enhanced by various factors such as IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, and IL17, while it is inhibited by glucocorticoids, IL4, and TGF beta. IL6 plays a direct role in responses following infection and cellular damage and could be as crucial as IL1 and TNF alpha in regulating the acute phase response. Moreover, IL6 is implicated in the regulation of adipose tissue mass.
Fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells are reported to produce IL6. It exerts its effects on a diverse array of cells including fibroblasts, myeloid progenitor cells, T cells, B cells, and hepatocytes. Furthermore, IL6 seems to interact with IL2 in T lymphocyte proliferation and enhances the proliferative effects of IL3 on multipotent hematopoietic progenitors. |
