| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Mouse |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This IL-6 antibody is un-conjugated |
| Application: |
ELISA, western blotting (WB) |
| Specificity: |
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-6 protein. A BLAST analysis was used to suggest cross-reactivity with IL-6 from mouse sources based on 100% homology with the immunizing sequence. Based on 94% homology, there is a chance of cross-reactivity to IL-6 from rat. Cross-reactivity with IL-6 from other sources has not been determined. |
| Characteristics: |
Interleukin (IL)-6, also know as BCDG, BCGF and BSF-2, is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Anti-IL-6 antibody is ideal for investigators involved in cancer, neuroscience and immunology research. |
| Purification: |
purified |
| Immunogen: |
Anti-IL-6 is an IgG fraction antibody prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E. coli. Immunogen type: Recombinant protein. |
| Isotype: |
IgG |
| Format: |
Lyophilized |
| Concentration: |
1.0 mg/mL |
| Reconstitution: |
Reconstitution buffer: restore with deionized water (or equivalent), Reconstitution volume: 100 µL |
| Buffer: |
0.02 M potassium phosphate, 0.15 M sodium chloride, pH7.2. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at - 20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six months from date of opening. |
| Expiry Date: |
6 months |
| Alternative Name: |
IL6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
Interleukin-6 (IL-6), known as BCDG, BCGF, and BSF-2, stands as a pivotal proinflammatory and immunoregulatory cytokine produced by diverse cell types. Studies have revealed its ability to hinder the growth of nascent melanoma cells while fostering the expansion of advanced-stage melanoma cells in experimental settings. |
