| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 34.8 kDa with 6×His-tag and a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 307 amino acids. |
| AA Sequence: |
MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHHC |
| Purity: |
> 96% by SDS-PAGE and RP-HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from additive free solution. |
| Reconstitution: |
Dissolve in distilled water or saline. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Protein A, found in the cell walls of various strains of Staphylococcus aureus, is comprised of a solitary polypeptide chain with minimal to no carbohydrate content. Recombinant Protein A, synthesized in E. coli, mirrors the functionality of its natural counterpart, featuring a sequential arrangement of 5 IgG-binding domains: E-D-A-B-C. |
| Shipping: |
The product is shipped at ambient temperature. Upon receipt, store it immediately at -20°C. |
| Concentration for 1 unity OD at 280 nm: |
4.589 mg/mL |
| Specificity: |
The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat. |
| Endotoxin Level: |
Less than 0.1 EU/μg of Protein A-Cys, His as determined by LAL method. |
