| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 33.5 kDa with a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 297 amino acids. |
| AA Sequence: |
NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDC |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from additive free solution. |
| Reconstitution: |
Dissolve in distilled water or saline. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Protein A, found in the cell walls of various strains of Staphylococcus aureus, is comprised of a solitary polypeptide chain with minimal to no carbohydrate content. Recombinant Protein A, synthesized in E. coli, mirrors the functionality of its natural counterpart, featuring a sequential arrangement of 5 IgG-binding domains: E-D-A-B-C. |
| Shipping: |
The product is shipped at ambient temperature. Upon receipt, store it immediately at -20°C. |
| Concentration for 1 unity OD at 280 nm: |
4.42 mg/mL |
| Specificity: |
The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat. |
| Endotoxin Level: |
Less than 0.1 EU/μg of Protein A-Cys as determined by LAL method. |
