| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 47.8 kDa with a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 430 amino acids. |
| AA Sequence: |
NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEC |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from additive free solution. |
| Reconstitution: |
Dissolve in distilled water or saline. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
The recombinant Protein A/G encompasses seven IgG-binding domains, designated as EDABC-C1C3, representing segments from both Protein A and Protein G included in its sequence. The Protein A segment comprises segments E, D, A, B, and C from Staphylococcus aureus, while the Protein G segment is derived from Streptococcus segments C1 and C3. Notably, the binding capacity of recombinant Protein A/G surpasses that of either Protein A or Protein G individually. |
| Shipping: |
The product is shipped at ambient temperature. Upon receipt, store it immediately at -20°C. |
| Concentration for 1 unity OD at 280 nm: |
1.737 mg/mL |
| Specificity: |
The recombinant Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G to ensure the maximum specific IgG binding. The recombinant Protein A/G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A/G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat. |
| Endotoxin Level: |
Less than 0.1 EU/μg of Protein A/G-Cys as determined by LAL method. |
