| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 41.5 kDa with a 6×His-tag on N-terminus, a single non-glycosylated polypeptide chain containing 372 amino acids. But it migrates with an apparent molecular mass of 46 kDa in SDS-PAGE. |
| AA Sequence: |
MHHHHHHKEE TPETPETDSE EEVTIKANLI FANGSTQTAE FKGTFEKATS EAYAYADTLK KDNGEYTVDV ADKGYTLNIK FAGKEKTPEE PKEEVTIKAN LIYADGKTQT AEFKGTFEEA TAEAYRYADA LKKDNGEYTV DVADKGYTLN IKFAGKEKTP EEPKEEVTIK ANLIYADGKT QTAEFKGTFE EATAEAYRYA DLLAKENGKY TVDVADKGYT LNIKFAGKEK TPEEPKEEVT IKANLIYADG KTQTAEFKGT FAEATAEAYR YADLLAKENG KYTADLEDGG YTINIRFAGK KVDEKPEEKE QVTIKENIYF EDGTVQTATF KGTFAEATAE AYRYADLLSK EHGKYTADLE DGGYTINIRF AG |
| Purity: |
> 97% by SDS-PAGE and SEC-HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from additive free solution. |
| Reconstitution: |
Dissolve in distilled water or saline. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Recombinant Protein L contains 5 kappa-binding domain. Protein L, equipped with five kappa-binding domains, possesses a distinctive capacity to engage with kappa light chains, all the while leaving the antibody's antigen-binding site untouched. This unique feature enables Protein L to interact with a broader spectrum of Ig classes and subclasses compared to alternative antibody-binding proteins. |
| Concentration for 1 unity OD at 280 nm: |
1.16 mg/mL |
| Specificity: |
The recombinant Protein L is a genetically engineered protein containing 5 IgG-binding regions of protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein L to ensure the maximum specific IgG binding. The recombinant Protein L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein L binds to human, mouse, rat and pig IgG. |
| Endotoxin Level: |
Less than 0.1 EU/μg of Protein L, His as determined by LAL method. |
