| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 40.5 kDa, a single non-glycosylated polypeptide chain containing 365 amino acids. But it migrates with an apparent molecular mass of 45 kDa in SDS-PAGE. |
| AA Sequence: |
KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAG |
| Purity: |
> 97% by SDS-PAGE and SEC-HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from additive free solution. |
| Reconstitution: |
Dissolve in distilled water or saline. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Recombinant Protein L contains 5 kappa-binding domain. Protein L, equipped with five kappa-binding domains, possesses a distinctive capacity to engage with kappa light chains, all the while leaving the antibody's antigen-binding site untouched. This unique feature enables Protein L to interact with a broader spectrum of Ig classes and subclasses compared to alternative antibody-binding proteins. |
| Concentration for 1 unity OD at 280 nm: |
1.133 mg/mL |
| Specificity: |
The recombinant Protein L is a genetically engineered protein containing 5 IgG-binding regions of protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein L to ensure the maximum specific IgG binding. The recombinant Protein L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein L binds to human, mouse, rat and pig IgG. |
| Endotoxin Level: |
Less than 0.1 EU/μg of Protein L as determined by LAL method. |
