| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 28.9 kDa, a single non-glycosylated polypeptide chain containing 264 amino acids. |
| AA Sequence: |
MHPETLVKVK DAEDQLGARV GYIELDLNSG KILESFRPEE RFPMMSTFKV LLCGAVLSRV DAGQEQLGRR IHYSQNDLVE YSPVTEKHLT DGMTVRELCS AAITMSDNTA ANLLLTTIGG PKELTAFLHN MGDHVTRLDR WEPELNEAIP NDERDTTMPA AMATTLRKLL TGELLTLASR QQLIDWMEAD KVAGPLLRSA LPAGWFIADK SGAGERGSRG IIAALGPDGK PSRIVVIYTT GSQATMDERN RQIAEIGASL IKHW |
| Purity: |
> 95% by SDS-PAGE. |
| Biological Activity: |
Fully biologically active when compared to standard. One unit of enzyme activity is defined as the amount of enzyme which will hydrolyze 1.0 μmol of benzyl penicillin in presence of EDTA at pH7.0 and at 25°C. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 100 mM Tris, pH7.0. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Beta-lactamases are enzymes produced by certain bacteria that enable them to resist beta-lactam antibiotics, including penicillins, cephamycins, and carbapenems. These enzymes deactivate the antibiotics by breaking the β-lactam ring, a four-atom structure essential to the antibiotics' antibacterial activity. The most frequently encountered beta-lactamase in gram-negative bacteria is TEM-1, which is responsible for up to 90% of ampicillin resistance in E. coli. TEM-1 also contributes to the growing resistance to ampicillin and penicillin observed in H. influenzae and N. gonorrhoeae. There are currently 140 known TEM-type enzymes, each characterized by different combinations of mutations. Recombinant TEM-1 beta-lactamase consists of 264 amino acid residues. |
