| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| AA Sequence: |
GPVAAVVREL RCVCLTTTPG IHPKTVSDLQ VIAAGPQCSK VEVIATLKNG REVCLDPEAP LIKKIVQKIL DSGKNN |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH7.0. |
| Endotoxin: |
Less than 0.1 EU/µg of rBoGCP-2/CXCL6 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
GCP-2 (granulocyte chemotactic protein-2) is a member of the CXC chemokine family. Among the human CXC chemokines, GCP-2 is most closely related to ENA-78, with both genes showing significant structural and sequence similarity. This similarity suggests that they may have arisen from a recent gene duplication event. In mice, a gene initially identified as LIX (LPS-induced CXC chemokine) was discovered to be induced by LPS in fibroblasts. The mouse protein referred to as GCP-2 shares 60% amino acid sequence similarity with human GCP-2, making it identical to the LIX protein sequence. |
