| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 9.5 kDa, a single non-glycosylated polypeptide chain containing 88 amino acids. |
| AA Sequence: |
ESSFPATFVP LPADSEGGED EDLQCVCLKT TSGINPRHIS SLEVIGAGTH CPSPQLLATK KTGRKICLDQ QRPLYKKILK KLLDGDES |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH7.0. |
| Endotoxin: |
Less than 0.1 EU/μg of rBoPF-4/CXCL4 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Platelet Factor-4 (PF-4) is a small cytokine within the CXC chemokine family, also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from the alpha granules of activated platelets during platelet aggregation and works to neutralize the anticoagulant effects of heparin. It interacts with a splice variant of the chemokine receptor CXCR3 and acts as a chemotactic agent for neutrophils, fibroblasts, and monocytes. Recombinant bovine CXCL4 consists of 88 amino acids. Notably, bovine and sheep CXCL4 share approximately 85% identity. |
