| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| AA Sequence: |
QPDSVSIPIT CCFSMVKRKI PMQKLESYMR ITNSQCPQEA VIFKTKASRE ICADPKQKWV QDYMNHLDQK SQAQKP |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 0.1 EU/μg of rCaMCP-2/CCL8 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. These proteins belong to the C-C chemokine family, with MCP-2 and MCP-3 sharing 62% and 71% amino acid sequence identity with MCP-1, respectively. Additionally, MCP-3 has 58% amino acid identity with MCP-2. Like other C-C chemokines, MCP-1, MCP-2, and MCP-3 function as monocyte chemoattractants. They also attract activated NK cells, as well as CD4+ and CD8+ T lymphocytes. Moreover, these cytokines have been shown to attract eosinophils and induce histamine secretion from basophils. |
