| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 39.9 kDa, a homodimer, non-glycosylated polypeptide chain containing 2 × 189 amino acids with Met, Gly and 10 × His at N-terminus. |
| AA Sequence: |
MGHHHHHHHH HHSSGHIEGR HMTYARAAAR QARALEATKA VCVLKGDGPV QGIINFEQKE SNGPVKVWGS IKGLTEGLHG FHVHEFGDNT AGCTSAGPHF NPLSRKHGGP KDEERHVGDL GNVTADKDGV ADVSIEDSVI SLSGDHCIIG RTLVVHEKAD DLGKGGNEES TKTGNAGSRL ACGVIGIAQ |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The potency per mg was determined by pyrogallol autoxidation method and was found to be more than 1600 U/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuCu/Zn SOD, His as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
SOD1 |
| Background: |
Superoxide dismutase facilitates the conversion of superoxide anions and hydrogen into molecular oxygen and hydrogen peroxide. In humans, Cu/Zn superoxide dismutase, also known as SOD1, is encoded by the SOD1 gene located on chromosome 21. This enzyme binds copper and zinc ions and is one of three SOD enzymes responsible for neutralizing free superoxide radicals in the body. SOD1 has been observed to interact with CCS and Bcl-2. Dysfunctions in SOD1 have been linked to increased susceptibility to conditions such as sarcopenia (age-related muscle mass loss), early onset of cataracts, macular degeneration, thymic involution, hepatocellular carcinoma, shortened lifespan, keratoconus, and amyotrophic lateral sclerosis. |
