| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids. |
| AA Sequence: |
CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
Supplied as a 0.2 μm filtered solution in PBS, pH7.4, with 1 mM DTT, 10% glycerol. |
| Endotoxin: |
Less than 0.1 EU/μg of rHuCyP-D as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70°C as supplied.- 3 months, -20 to -70°C under sterile conditions after opening. |
| Synonyms: |
PPIF, Cyclophilin F, CyP-M, Rotamase F |
| Background: |
CyP-D belongs to the peptidyl-prolyl cis-trans isomerase (PPIase) family and plays a crucial role in protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It is a key component of the mitochondrial permeability transition pore located in the inner mitochondrial membrane. The activation of this pore is believed to contribute to the initiation of both apoptotic and necrotic cell death. |
