Recombinant Human EGF Protein (ECD), HPLC-Verified

2-1-1-green-tea-extract-1

Recombinant Human EGF Protein (ECD), HPLC-Verified

Cat. No.: SPOLEO00012
Epidermal growth factor (EGF) is the prototypical member of the EGF protein family, known for its highly similar structural and functional properties. EGF comprises nine EGF-like domains and nine LDL-receptor class B repeats. The human form of EGF is a 6,045-Da protein, consisting of 53 amino acids and featuring three intramolecular disulfide bonds. Initially purified from the mouse submandibular gland, EGF has since been identified in various human tissues, including the submandibular and parotid glands. It is also present in human platelets, macrophages, urine, saliva, milk, and plasma. EGF functions as a growth factor, promoting the growth of various epidermal and epithelial tissues both in vivo and in vitro, as well as stimulating the proliferation of some fibroblasts in cell culture. Its effects include cellular proliferation, differentiation, and survival.
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Description: Epidermal growth factor (EGF) is the prototypical member of the EGF protein family, known for its highly similar structural and functional properties. EGF comprises nine EGF-like domains and nine LDL-receptor class B repeats. The human form of EGF is a 6,045-Da protein, consisting of 53 amino acids and featuring three intramolecular disulfide bonds. Initially purified from the mouse submandibular gland, EGF has since been identified in various human tissues, including the submandibular and parotid glands. It is also present in human platelets, macrophages, urine, saliva, milk, and plasma. EGF functions as a growth factor, promoting the growth of various epidermal and epithelial tissues both in vivo and in vitro, as well as stimulating the proliferation of some fibroblasts in cell culture. Its effects include cellular proliferation, differentiation, and survival.
Purity: ≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.
Protein Construction: A DNA sequence encoding the mature form of human EGF (Asn 971-Arg 1023) was expressed and purified with an initial Met at the N-terminus.
Expressed Host: E. coli
Species: Human
Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Formulation: Lyophilized from sterile PBS, pH7.4.
Endotoxin: < 5 EU per mg of the protein.

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