| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.3 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids. |
| AA Sequence: |
AGPAAAVLRE LRCVCLQTTQ GVHPKMISNL QVFAIGPQCS KVEVVASLKN GKEICLDPEA PFLKKVIQKI LDGGNKEN |
| Purity: |
> 97% by SDS-PAGE analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 5.0-10.0 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 500 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHuENA-78, 1-78a.a./CXCL5 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
CXCL5, also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78), is part of the CXC chemokine family. It is produced when cells are stimulated by inflammatory cytokines like interleukin-1 or tumor necrosis factor-alpha. ENA-78 (8-78) and ENA-78 (9-78) exhibit a threefold increase in chemotactic activity for neutrophil granulocytes in vitro, generated by proteolytic cleavage after secretion from peripheral blood monocytes. Recombinant human CXCL5 (1-78 a.a.) consists of 78 amino acids in a single non-glycosylated polypeptide chain. Human CXCL5 has 57% amino acid sequence identity with its mouse and rat counterparts. |
