| Synonym(s): |
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin |
| Description: |
FGF basic is a member of the FGF family, a group of over 23 mitogenic proteins sharing 35-60% amino acid similarity. Unlike typical FGFs, FGF acidic and basic lack signal peptides and are secreted through unconventional pathways. FGF basic, isolated from various tissues including neural tissue and placenta, contains four cysteine residues but retains full biological activity even when disulfide bonds are reduced.
It binds to heparin or cell surface heparan sulfate proteoglycans to interact with high-affinity FGF receptors, similar to FGF acidic. FGF basic stimulates proliferation in mesodermal-origin cells and many from neuroectodermal, ectodermal, and endodermal origins. It also induces neuron differentiation, survival, and regeneration.
In vitro studies suggest FGF basic plays roles in embryonic development, angiogenesis, wound healing, tissue repair, and neuronal function. Its activities imply involvement in pathological conditions characterized by excessive cell proliferation and angiogenesis. |
| Source: |
E. coli-derived human FGF basic/FGF2/bFGF protein
Gly132-Ser288 |
| Purity: |
> 97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Shipping: |
Shipped at ambient temperature |
| Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Reconstitution: |
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitute at 100 μg/mL in sterile PBS. |
| Form: |
Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl. |
| Predicted Molecular Mass: |
17.4 kDa |
| Endotoxin: |
< 0.10 EU per 1 μg of the protein by the LAL method. |
| N-terminal Sequence Analysis: |
Gly132 |
