| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 26.0 kDa, a single non-glycosylated polypeptide chain containing 249 amino acids. |
| AA Sequence: |
ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYPGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSATGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by its ability to agglutinate human red blood cells is less than 10 μg/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in 1×PBS, 5% trehalose, 0.02% Tween-20, 3mM β-ME, pH7.4. |
| Endotoxin: |
Less than 0.1 EU/µg of rHuGalectin-3 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
AGE-R3, CBP35, GAL3, L29, LGALS3, Mac-2 |
| Background: |
Human galectin-3, also known as AGE-R3, CBP35, GAL3, L29, LGALS3, and Mac-2, belongs to the galectin family and is encoded by the LGALS3 gene located on chromosome 14 at locus q21–q22. It is found in the nucleus, cytoplasm, mitochondria, cell surface, and extracellular space. Galectin-3 has a molecular weight of approximately 30 kDa and contains a carbohydrate-recognition-binding domain (CRD) of about 130 amino acids, which allows it to specifically bind to β-galactosides. Due to its wide range of biological functions, Galectin-3 is involved in various conditions, including cancer, inflammation, fibrosis, heart disease, and stroke. Research indicates that Galectin-3 expression plays a role in several processes related to heart failure, such as myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and ventricular remodeling. Human galectin-3 shares 79% amino acid sequence identity with its counterparts in rats and mice. |
