| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 22 kDa, a single non-glycosylated polypeptide chain containing 191 amino acids |
| AA Sequence: |
FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution with 20mM PB, pH7.0, with 3% Mannitol. |
| Endotoxin: |
Less than 1 EU/µg of rHuGH as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
GH1, GH, GHN, GH-N, hGH-N,Pituitary Growth Hormone, Growth Hormone 1, Somatotropin. |
| Background: |
Growth hormone (GH), also referred to as somatotropin, belongs to a group of growth factors that encompass prolactin, placental lactogens, proliferins, and somatolactin. This peptide hormone is pivotal in promoting growth, cellular reproduction, and regeneration across humans and other species. Comprising a 191-amino acid, single-chain polypeptide, GH is produced, stored, and released by somatotropic cells situated in the anterior pituitary gland's lateral wings. Its intermittent entry into the bloodstream is controlled by the synergistic effects of hypothalamic hormones—GH-releasing hormone (GHRH) and somatostatin (SST)—alongside peripheral signals such as ghrelin and leptin. The human GH gene produces a precursor protein of 217 amino acids, which includes a 26 amino acid signal peptide presumed to guide secretion. Through the process of alternative splicing, a minimum of four GH variants have been discovered. |
