| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids. |
| AA Sequence: |
IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS with 0.02% Tween-20, pH7.0. |
| Endotoxin: |
Less than 1 EU/µg of rHuIHH C28II as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution. |
| Synonyms: |
HHG-2 |
| Background: |
IHH, encoded by the IHH gene in humans, is part of the mammalian hedgehog family. It is expressed in the adult kidney and liver. IHH plays a crucial role in chondrocyte differentiation, proliferation, and maturation, particularly during endochondral ossification. It exerts its effects through the feedback regulation of parathyroid hormone-related peptide (PTHrP). Additionally, IHH is involved in yolk sac vasculogenesis, significantly contributing to the differentiation of epiblast cells into endothelial and red blood cells. The human IHH protein shares 100% amino acid sequence identity with its murine counterpart. |
