| Source: |
Insect Cell |
| Molecular Weight: |
Approximately 104.7 kDa on SDS-PAGE under reducing conditions, containing 942 amino acids. |
| AA Sequence: |
AGMGMLQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLTPNQQRISVVWNDDSSSTLTIYNANIDDAGIYKCVVTGEDGSESEATVNVKIFQKLMFKNAPTPQEFREGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTIQARQNIVNATANLGQSVTLVCDAEGFPEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKKVDKNDEAEYICIAENKAGEQDATIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDQVEPYSSTAQVQFDEPEATGGVPILKYKAEWRAVGEEVWHSKWYDAKEASMEGIVTIVGLKPETTYAVRLAALNGKGLGEISAASEFKTQPVQGEPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVRYRALSSEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTSGIEGRMDEPKSSDKTHTCPPCPAPEFEGAPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPTPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| Purity: |
> 90% by SDS-PAGE analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in PBS, pH7.0, with 5% Trehalose, 0.02% Tween-20. |
| Endotoxin: |
Less than 0.1 EU/μg of rHuNCAM-1/CD56-Fc as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Neural cell adhesion molecule 1 (NCAM-1) is a versatile member of the Ig superfamily, playing crucial roles in various cellular interactions. Belonging to a group of membrane-bound glycoproteins, it engages in Ca++-independent cell-matrix interactions as well as homophilic or heterophilic cell-cell adhesions. NCAM-1 exhibits specific binding capabilities to heparan sulfate proteoglycans, agrin, and several chondroitin sulfate proteoglycans including neurocan and phosphocan. Human NCAM-1 manifests in three principal forms resulting from alternate splicing: NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa), where NCAM-120 is GPI-linked, while NCAM‑140 and NCAM-180 are type I transmembrane glycoproteins. Extensive alternate splicing and possible extracellular proteolytic processing further diversify all three forms. Synthesized as a 761 aa preproprecursor with a 19 aa signal sequence, NCAM-1 consists of a 722 aa GPI-linked mature region and a 20 aa C-terminal prosegment, featuring five C-2 type Ig-like domains and two fibronectin type-III domains. Human and mouse NCAM-1 share 93% aa identity, with NCAM-1 being highly sialylated. Polysialylation of NCAM-1 diminishes its adhesive properties while enhancing neurite outgrowth promotion. Notably, the adult brain displays reduced sialylation of NCAM-1 compared to earlier developmental stages, yet regions exhibiting heightened neuronal plasticity maintain polysialylation-NCAM-1, indicating its involvement in regenerative processes and synaptic plasticity. |
