| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 47.1 kDa, a single non-glycosylated polypeptide chain containing 433 amino acids. |
| AA Sequence: |
SIEKIWAREI LDSRGNPTVE VDLYTAKGLF RAAVPSGAST GIYEALELRD GDKQRYLGKG VLKAVDHINS TIAPALISSG LSVVEQEKLD NLMLELDGTE NKSKFGANAI LGVSLAVCKA GAAERELPLY RHIAQLAGNS DLILPVPAFN VINGGSHAGN KLAMQEFMIL PVGAESFRDA MRLGAEVYHT LKGVIKDKYG KDATNVGDEG GFAPNILENS EALELVKEAI DKAGYTEKIV IGMDVAASEF YRDGKYDLDF KSPTDPSRYI TGDQLGALYQ DFVRDYPVVS IEDPFDQDDW AAWSKFTANV GIQIVGDDLT VTNPKRIERA VEEKACNCLL LKVNQIGSVT EAIQACKLAQ ENGWGVMVSH RSGETEDTFI ADLVVGLCTG QIKTGAPCRS ERLAKYNQLM RIEEELGDEA RFAGHNFRNP SVL |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4, with 5% Trehalose. |
| Endotoxin: |
Less than 0.1 EU/µg of rHuNSE as determined by LAL method. |
| Background: |
Neuron-specific enolase (enolase 2) is a 47.1 kDa enzyme belonging to the enolase family, expressed in developing neurons and glia. It catalyzes phosphoenolpyruvate formation and is thought to promote neuronal survival, possibly through extracellular mechanisms. Human Neuron-specific enolase comprises 433 amino acids (aa) and its enzymatic activity spans most of its length. It can exist as a noncovalently-linked homodimer or form a heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with its murine and canine counterparts and 83% aa identity with human enolases 1 and 3. |
