| Source: |
Mouse myeloma cell line, NS0 |
| Molecular Weight: |
Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6× polyhistidine tag. |
| AA Sequence: |
Ile17-Asn300, with a C-terminal 6-His tag; Accession # NP_000573.1 |
| Purity: |
> 95% by SDS-PAGE analyses. |
| Biological Activity: |
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 × 105 cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37°C. The ED50 for this effect is typically 0.1-0.6 μg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from 0.2 µm filtered concentrated solution in PBS, pH7.2. |
| Endotoxin: |
Less than 0.1 EU/µg of rHuOsteopontin/OPN as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Further dilutions should be made in appropriately buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Osteopontin (OPN), also known as SPP1, Eta-1, or BSP, is a highly acidic secreted protein ranging from 45-75 kDa within the SIBLING family of matricellular proteins. It undergoes various modifications, including O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN interacts with several integrins and can engage additional integrins after proteolytic cleavage. It is predominantly expressed in mineralized tissues, where it plays a role in inhibiting bone mineralization and kidney stone formation while promoting inflammation, cell adhesion, and cell migration. OPN expression is elevated during states of inflammation, obesity, atherosclerosis, cancer, and tissue damage, contributing to the underlying pathology of these conditions. |
