| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 9551 Da, a single non-glycosylated polypeptide chain containing 84 amino acids. 15N stable isotope labeled. |
| AA Sequence: |
SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 104 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuPTH1-84, 15N as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Parathormone, Parathyrin |
| Background: |
Parathyroid hormone (PTH) consists of 84 amino acids and plays a crucial role in maintaining calcium (Ca2+) balance in the body. Produced by the parathyroid glands, PTH stimulates the release of calcium from bone by activating osteoblasts and inhibiting osteoclasts. It also enhances calcium absorption in the intestines and promotes renal tubular reabsorption of calcium while increasing phosphate excretion. PTH is a key regulator of bone metabolism and its secretion rises in response to low extracellular fluid calcium levels. |
