| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 26.1 kDa, a single non-glycosylated polypeptide chain containing 237 amino acids. |
| AA Sequence: |
IVGGWECEKH SQPWQVLVAS RGRAVCGGVL VHPQWVLTAA HCIRNKSVIL LGRHSLFHPE DTGQVFQVSH SFPHPLYDMS LLKNRFLRPG DDSSHDLMLL RLSEPAELTD AVKVMDLPTQ EPALGTTCYA SGWGSIEPEE FLTPKKLQCV DLHVISNDVC AQVHPQKVTK FMLCAGRWTG GKSTCSGDSG GPLVCNGVLQ GITSWGSEPC ALPERPSLYT KVVHYRKWIK DTIVANP |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH8.0, 150 mM NaCl, 3% trehalose. |
| Endotoxin: |
Less than 0.1 EU/μg of rHuPSA/Kallikrein-3 as determined by LAL method. |
| Background: |
The human tissue kallikrein (KLK) gene family comprises 15 members with significant implications in cancer. Among them, kallikrein-3, known as prostate-specific antigen (PSA), is a glycoprotein enzyme encoded by the KLK3 gene in humans. PSA is produced by prostate gland epithelial cells and functions by hydrolyzing semenogelin-1, which facilitates the liquefaction of the seminal coagulum. PSA serves as a well-established tumor marker crucial for diagnosing, staging, and monitoring prostate cancer. |
