| Synonym(s): |
Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1 |
| Purity: |
> 90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining. |
| Endotoxin Level: |
< 0.10 EU per 1 μg of the protein by the LAL method |
| Activity: |
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.00-10.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a |
| Source: |
Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein
Ser21-Ala263 |
| Accession #: |
Q2MKA7 |
| N-terminal Sequence Analysis: |
Ser21 & Arg31 |
| Predicted Molecular Mass: |
25.6 kDa |
| SDS-PAGE: |
39 kDa, reducing conditions |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution: |
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping: |
Shipp at ambient temperature |
| Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Entrez Gene IDs: |
284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey) |
