| Source: |
Escherichia coli |
| Molecular Weight: |
The protein has a calculated MW of 23.9 kDa, containing 232 amino acids. The protein migrates as 40-50 kDa in SDS-PAGE under reducing condition. |
| AA Sequence: |
QIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLG |
| Purity: |
> 95% by SDS-PAGE analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.0. |
| Endotoxin: |
Less than 0.1 EU/μg of rHuSyndecan-1/CD138 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Syndecan-1 (CD138) is a type I transmembrane protein in the syndecan family, carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs). It forms weak non-covalent homodimers or heterodimers with Syndecan-2 or -3 through the transmembrane domain. Synthesized as a 310 amino acid precursor, it includes a 17 aa signal sequence, a 234 aa extracellular domain (ECD) with three Ser-Gly HS attachment sites, a 25 aa transmembrane segment, and a 34 aa cytoplasmic region with a PDZ binding motif and tyrosine phosphorylation site. The ECD is modified by GAGs, resulting in molecular weights of 120-200 kDa. Soluble forms are shed via proteolytic cleavage. The human Syndecan-1 ECD shares 65-71% aa identity with those of rat, mouse, canine, equine, and bovine Syndecan-1.
Syndecan-1 is highly expressed in epithelial cells and terminally differentiated B cells like plasma cells. It aids wound healing in the skin, cornea, and heart by promoting re-epithelialization, migration, and collagen deposition. It binds chemokines to create chemotactic gradients and modulates integrins to control leukocyte influx, limiting inflammation. In cancers, the shedding of Syndecan-1 can facilitate growth, angiogenesis, and metastasis. Growth factors like FGFs and HGF bind to its GAG chains, using Syndecan-1 as a coreceptor. These GAG chains also help viruses and bacteria in cell adhesion and uptake. |
