| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 19.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids (a.a.) of human UBE2I/UBC9 and 8 a.a. vector sequence including 6 × His tag at N-terminus. |
| AA Sequence: |
MHHHHHHAMG TLNMSGIALS RLAQERKAWR KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP S |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH 7.6, with 125 mM NaCl, 10% glycerol, 1 mM DTT. |
| Endotoxin: |
Less than 1 EU/µg of rHuUBE2I/UBC9, His as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70°C as supplied.- 3 months, -20 to -70°C under sterile conditions after opening. |
| Synonyms: |
SUMO-protein Ligase, Ubiquitin Carrier Protein 9, Ubiquitin Carrier Protein I, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, p18 |
| Background: |
Ubiquitin-conjugating enzyme E2 I (UBE2I), also known as ubiquitin-conjugating Enzyme 9 (UBC9), is part of the ubiquitin-conjugating enzyme family and is encoded by the UBE2I gene in humans. These enzymes also referred to as E2 enzymes or occasionally as ubiquitin-carrier enzymes, play a crucial role in the second step of the ubiquitination process. In this process, E1 enzymes activate ubiquitin by attaching it covalently to their active site cysteine residue. The activated ubiquitin is then transferred to the cysteine residue of an E2 enzyme. Subsequently, the E2 enzyme interacts with an E3 ligase through a conserved binding region. UBE2I/UBC9 specifically accepts ubiquitin-like proteins SUMO1-4 from the UBLE1A-UBLE1B E1 complex and, with the assistance of an E3 ligase such as RANBP2 or CBX4, catalyzes their covalent attachment to target proteins. Additionally, UBE2I/UBC9 is involved in the formation of poly-SUMO chains, the sumoylation of FOXL2 and KAT5, and the regulation of nuclear architecture and chromosome segregation. |
