| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 19.0 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids of human UBE2B and 14 a.a. vector sequence including 6 × His tag at N-terminus. |
| AA Sequence: |
MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH7.6 with 125 mM NaCl, 10% glycerol, 5% trehalose, 1 mM DTT. |
| Endotoxin: |
Less than 1 EU/µg of rHuUBE2B, His as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70°C as supplied.- 3 months, -20 to -70°C under sterile conditions after opening. |
| Synonyms: |
HR6B, Ubiquitin Carrier Protein B, Ubiquitin-protein Ligase B |
| Background: |
Ubiquitin-conjugating enzyme E2 B, encoded by the UBE2B gene in humans, belongs to the ubiquitin-conjugating enzyme family. These enzymes, also known as E2 enzymes or ubiquitin-carrier enzymes, play a crucial role in the second step of the ubiquitination process. Initially, E1 activates ubiquitin by attaching it covalently to its active site cysteine residue. Subsequently, the activated ubiquitin is transferred to a cysteine residue on E2, which then interacts with E3 through a structurally conserved binding region. This ubiquitination reaction modifies proteins and regulates protein degradation. UBE2B specifically interacts with RAD18, UBR2, and WAC proteins. Its protein sequence is identical across mouse, rat, and rabbit homologs, underscoring its high conservation throughout eukaryotic evolution. |
