| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids (a.a.) of human UBE2C/UBCH10 and 12 a.a. vector sequence including 6 × His tag at N-terminus. |
| AA Sequence: |
MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE P |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH8.0, with 125 mM NaCl, 10% glycerol, 5% trehalose, 1 mM DTT. |
| Endotoxin: |
Less than 1 EU/µg of rHuUBE2C, His as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70°C as supplied.- 3 months, -20 to -70°C under sterile conditions after opening. |
| Synonyms: |
UbcH10, Ubiquitin Carrier Protein C, Ubiquitin-protein Ligase C |
| Background: |
Ubiquitin-conjugating enzyme E2 C, encoded by the UBE2C gene in humans, is a member of the ubiquitin-conjugating enzyme family. These enzymes, also known as E2 enzymes or ubiquitin-carrier enzymes, play a crucial role in the second step of ubiquitination. Initially, E1 activates ubiquitin by attaching it covalently to its active site cysteine residue. The activated ubiquitin is subsequently transferred to a cysteine residue on an E2 enzyme, which then binds to an E3 ligase via a conserved binding region.
UBE2C specifically facilitates the degradation of cyclins A and B in coordination with the anaphase-promoting complex (APC), thereby regulating exit from mitosis. This function is critical at the end of mitosis to deactivate Cdc2 kinase and transition cells from mitosis to the G1 phase of the next cell cycle. Additionally, UBCH10, the human homolog of yeast PAS2, which is essential for peroxisome biogenesis, is utilized in vitro for ubiquitination reactions. |
