| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids (a.a.) of human UBE2D3/UBC5C and 8 a.a. vector sequence including 6 × His tag at N-terminus. |
| AA Sequence: |
MHHHHHHAMA LKRINKELSD LARDPPAQCS AGPVGDDMFH WQATIMGPND SPYQGGVFFL TIHFPTDYPF KPPKVAFTTR IYHPNINSNG SICLDILRSQ WSPALTISKV LLSICSLLCD PNPDDPLVPE IARIYKTDRD KYNRISREWT QKYAM |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH6.5, with 125 mM NaCl, 10% glycerol, 5% trehalose, 1 mM DTT. |
| Endotoxin: |
Less than 1 EU/µg of rHuUBE2D3/UBC5C, His as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70°C as supplied.- 3 months, -20 to -70°C under sterile conditions after opening. |
| Synonyms: |
Ubiquitin Carrier Protein D3, Ubiquitin-protein Ligase D3 |
| Background: |
Ubiquitin-conjugating enzyme E2 D3, encoded by the UBE2D3 gene in humans, is a member of the ubiquitin-conjugating enzyme family. These enzymes, also known as E2 enzymes or ubiquitin-carrier enzymes, play a crucial role in the ubiquitination process's second step. Initially, E1 activates ubiquitin by attaching it covalently to its active site cysteine residue. The activated ubiquitin is subsequently transferred to a cysteine residue on an E2 enzyme. E2 enzymes then interact with E3 ligases through a structurally conserved binding region. This ubiquitination process modifies proteins, thereby regulating protein degradation.
UBE2D3 is the human counterpart of the yeast UBC4/5 family and is involved in numerous regulatory functions linked to inflammation and cancer. It facilitates the degradation of various short-lived regulatory proteins, such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBα, and p105. UBE2D3 shares significant sequence identity, 88% with UbcH5a and 89% with UbcH5b, highlighting its structural and functional similarities to these related E2 enzymes. |
