| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids (a.a.) of human UBE2K and 8 a.a. vector sequence including 6 × His tag at N-terminus. |
| AA Sequence: |
MHHHHHHAMA NIAVQRIKRE FKEVLKSEET SKNQIKVDLV DENFTELRGE IAGPPDTPYE GGRYQLEIKI PETYPFNPPK VRFITKIWHP NISSVTGAIC LDILKDQWAA AMTLRTVLLS LQALLAAAEP DDPQDAVVAN QYKQNPEMFK QTARLWAHVY AGAPVSSPEY TKKIENLCAM GFDRNAVIVA LSSKSWDVET ATELLLSN |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH8.0, with 100 mM NaCl, 10% glycerol, 5% trehalose, 1 mM DTT. |
| Endotoxin: |
Less than 1 EU/µg of rHuUBE2K, His as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70°C as supplied.- 3 months, -20 to -70°C under sterile conditions after opening. |
| Synonyms: |
HIP-2, Ubiquitin Carrier Protein, Ubiquitin-protein Ligase |
| Background: |
Ubiquitin-conjugating enzyme E2 K, encoded by the UBE2K gene in humans, is a member of the ubiquitin-conjugating enzyme family. These enzymes, also known as E2 enzymes or ubiquitin-carrier enzymes, play a crucial role in the ubiquitination process as the second step. Initially, E1 activates ubiquitin by attaching it to its active site cysteine residue. Subsequently, the activated ubiquitin is transferred to a cysteine residue on E2, which then binds to E3 through a structurally conserved binding region. This ubiquitination process modifies proteins and regulates protein degradation pathways.
UBE2K has demonstrated interactions with Huntingtin and RNF2 proteins. Furthermore, it has been implicated in the degradation of huntingtin and in the suppression of apoptosis. |
