| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids (a.a.) of human UBE2L3/UBCH7 and 8 a.a. vector sequence including 6 × His tag at N-terminus. |
| AA Sequence: |
MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH7.0, with 125 mM NaCl, 10% glycerol, 5% trehalose, 1 mM DTT. |
| Endotoxin: |
Less than 1 EU/µg of rHuUBE2L3, His as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70°C as supplied.- 3 months, -20 to -70°C under sterile conditions after opening. |
| Synonyms: |
L-UBC, UbcH7, Ubiquitin Carrier Protein L3, Ubiquitin-protein Ligase L3 |
| Background: |
Ubiquitin-conjugating enzyme E2 L3, encoded by the UBE2L3 gene in humans, is a member of the ubiquitin-conjugating enzyme family. These enzymes, also known as E2 enzymes or ubiquitin-carrier enzymes, play a crucial role in the second step of ubiquitination. Initially, E1 activates ubiquitin by attaching it covalently to its active site cysteine residue. Subsequently, the activated ubiquitin is transferred to a cysteine residue on an E2 enzyme. The E2 molecule then binds to an E3 ligase via a conserved binding region.
UBE2L3 specifically interacts with HECT-type and RBR family E3 ubiquitin-protein ligases, including UBOX5, ARIH1, Cbl, UBE3A, and NEDD4. It is down-regulated during the S-phase of the cell cycle, influencing cell cycle progression. Additionally, UBE2L3 regulates the transcriptional activity of nuclear hormone receptors and plays a role in myelopoiesis. |
