Source: |
Escherichia coli |
Molecular Weight: |
Approximately 20.9 kDa, a single non-glycosylated polypeptide chain containing 183 amino acids. |
AA Sequence: |
MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK |
Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
Physical Appearance: |
Sterile filtered liquid. |
Formulation: |
A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH6.5, with 125 mM NaCl, 10% glycerol, 5% trehalose, 1 mM DTT. |
Endotoxin: |
Less than 0.1 EU/µg of rHuUBE2M as determined by LAL method. |
Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70°C as supplied.- 3 months, -20 to -70°C under sterile conditions after opening. |