| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 16.4 kDa, a single non-glycosylated polypeptide chain containing 142 amino acids. |
| AA Sequence: |
QELRCLCIQE HSEFIPLKLI KNIMVIFETI YCNRKEVIAV PKNGSMICLD PDAPWVKATV GPITNRFLPE DLKQKEFPPA MKLLYSVEHE KPLYLSFGRP ENKRIFPFPI RETSRHFADL AHNSDRNFLR DSSEVSLTGS DA |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 20-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4, with 0.02% Tween-20. |
| Endotoxin: |
Less than 1 EU/µg of rMuLungkine/CXCL15 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Mouse Lungkine/CXCL15, also known as WECHE, belongs to the ELR motif-containing CXC chemokines. The cDNA of mouse Lungkine encodes a protein consisting of 166 amino acids (aa), featuring a 25 aa predicted signal peptide and a 141 aa mature protein with an exceptionally long C-terminal tail extending beyond the chemokine fold. Mouse Lungkine exhibits 35% aa sequence identity with human ENA-78 and 31% identity with human IL-8. The gene encoding mouse Lungkine is located on chromosome 5. Northern blot and in situ hybridization techniques have shown that Lungkine transcripts are specifically detected in both adult and fetal lungs, with expression levels increasing under inflammatory conditions. Lungkine protein is secreted into the bronchoalveolar space and plays a role in lung-specific neutrophil trafficking. Studies involving Lungkine knockout mice indicate that Lungkine is crucial for neutrophil migration from the lung parenchyma into the airspace. Additionally, Lungkine acts as a chemoattractant for bone marrow progenitor cells and influences hematopoietic cell differentiation. |
