| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids. |
| AA Sequence: |
GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS |
| Purity: |
> 97% by SDS-PAGE analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 2×PBS, 5% Trehalose, 0.02% Tween-20, pH7.0. |
| Endotoxin: |
Less than 1 EU/μg of rMuMDC/CCL22 as determined by LAL method. |
| Reconstitution: |
Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
A minimum of 12 months from date of receipt, when stored at ≤ -20°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
STCP-1, MDC (1-69), Macrophage-derived Chemokine |
| Background: |
CCL22, encoded by the CCL22 gene located on Chromosome 8 in mice, exhibits high expression in macrophages, monocyte-derived dendritic cells, and the thymus. It is also detected in thymic, lymphatic, and appendiceal tissues. Acting as a chemoattractant, CCL22 binds to CCR4 and selectively attracts monocytes, monocyte-derived dendritic cells, and natural killer cells, while showing no attraction towards neutrophils, eosinophils, or resting T-lymphocytes. Following secretion from monocyte-derived dendritic cells, the protein undergoes proteolytic cleavage, resulting in three forms: MDC (3-69), MDC (5-69), and MDC (7-69). |
