| Description: |
Noggin is part of a group of soluble proteins that bind to TGF-β family ligands, regulating their activity by blocking their access to signaling receptors. Originally identified as an antagonist of BMP-4, Noggin is crucial for the proper formation of the head and other dorsal structures. It also modulates the activities of other BMPs such as BMP-2, BMP-7, BMP-13, and BMP-14. In mice, deletion of the noggin gene leads to prenatal death and a recessive phenotype characterized by severely malformed skeletal structures. Conversely, transgenic mice that overexpress noggin in mature osteoblasts exhibit impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant Murine Noggin is a 46.4 kDa homodimer linked by disulfide bonds, composed of two 206 amino acid polypeptide chains. |
| Purity: |
≥ 98% by SDS-PAGE gel and HPLC analyses. |
| Source: |
E.coli |
| Accession #: |
P97466 |
| Entrez Gene IDs: |
18121 |
| AA Sequence: |
MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC |
| Cross Reactivity Cited in References: |
Human, mouse |
| Biological Activity: |
Determined by its ability to inhibit 5.0 ng/mL of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells. The expected ED50 for this effect is 1.0-2.0 ng/mL of Noggin. |
| Calculated Molecular Weight: |
46.4 kDa |
| Endotoxin: |
Endotoxin level is < 0.1 ng/μg of protein (< 1 EU/μg) |
