In the development process of biologics for oral diseases, successful expression of heterologous genes is crucial for the development of important reagents and biomolecules. Alta Stomatology's recombinant protein expression platform offers customers customized services for recombinant proteins expression in various systems including Escherichia coli, Saccharomyces cerevisiae, and mammalian cells. Through overall project design, gene optimization, and expression condition optimization, high expression, high purity, and high activity recombinant proteins can be obtained. Leveraging rich technical experience and platform advantages, the company is capable of providing customers with customized services for high-purity recombinant proteins at milligram to gram scales. 

Platform Advantages

• Outstanding Protein Analysis Services

Through analysis of protein primary sequences and advanced structures, combined with downstream applications, we can design optimal segment recombinant proteins for you and select the most suitable expression systems.

• Comprehensive Expression Optimization Services

Based on the characteristics of the target sequence, we greatly improve gene expression levels and protein solubility through codon optimization and culture condition optimization.

• Multi-System Selection Guarantee Services

We have a complete expression system that can select the best expression system for different proteins. With our rich experience, we can provide guaranteed protein expression services.

• Integrated Upstream and Downstream Services

We can provide you with one-stop services from protein design to production, as well as corresponding series of quality inspection and downstream applications, such as antibody preparation, immunological testing, and animal evaluation.

Expression System Selection

When producing recombinant proteins, we need to choose the most suitable expression system based on the characteristics of the expression system and the intrinsic properties of the recombinant protein. Typically, the following factors need to be considered:

Protein Intrinsic Properties

These include molecular weight, hydrophilicity, structural complexity, species specificity, post-translational modification types, and subcellular localization of the protein.

Protein Demand Quantity

Different applications require different quantities of proteins. Research on protein functionality typically requires around 200 μg, while antibody preparation may require around 2-5 mg. For drug function studies, even larger quantities may be necessary.

Removal of Purification Tags

Common purification tags include His, GST, SUMO, MBP, etc., each with different characteristics. His tags, for instance, have a small molecular weight and generally do not affect the target protein significantly, while other tags may increase the molecular weight of the target protein while improving solubility. Whether to remove these tags depends on downstream applications.

Expression System Selection

Different expression systems have their own advantages, especially regarding post-translational modifications of proteins, which vary significantly among different expression systems. Therefore, the optimal expression system should be chosen based on the downstream applications of the protein.

Laboratory Equipment