| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids. |
| AA Sequence: |
HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rRtEotaxin/CCL11 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Small-inducible Cytokine A11 |
| Background: |
The CCL11 gene encodes a protein that is part of the CC chemokine family. CCL11 is a potent eosinophil chemoattractant, initially purified from the bronchoalveolar lavage fluid of guinea pigs exposed to ovalbumin aerosol. It is highly effective and specific in attracting eosinophils in vitro, without affecting monocytes or neutrophils. CCR3 is identified as the specific receptor for CCL11. Additionally, CCR3 acts as a cofactor for a specific subset of primary HIV viruses, and the binding of CCL11 to CCR3 has been shown to inhibit infection by these HIV isolates. |
