| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids. |
| AA Sequence: |
GPYGANVEDS ICCQDYIRHP LPPRFVKEFY WTSKSCRKPG VVLITIKNRD ICADPRMLWV KKILHKLA |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rRtMDC/CCL22 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
STCP-1, MDC (1-69), Macrophage-derived Chemokine |
| Background: |
CCL22 is a protein encoded by the CCL22 gene, prominently expressed in macrophages, monocyte-derived dendritic cells, and the thymus. It is also present in the thymus, lymph nodes, and appendix. CCL22 binds to the CCR4 receptor and acts as a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, or resting T-lymphocytes. Once secreted from monocyte-derived dendritic cells, CCL22 can be proteolytically cleaved into three forms: MDC (3-69), MDC (5-69), and MDC (7-69). |
