| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids. |
| AA Sequence: |
QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rRtMCP-3/CCL7 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Protein FIC, Small-inducible Cytokine A7, Intercrine/chemokine MARC |
| Background: |
Rat CCL7, part of the CC chemokine family, is also known as MCP-3. Sequence comparisons indicate that rat CCL7 is homologous to human MCP-3, leading to its identification as rat MCP-3. The MCP-3 protein family primarily signals through CCR2, with the exception of MCP-1, and demonstrates cross-species reactivity. CCL7/MCP-3 exhibits chemotactic properties for monocytes and eosinophils, but not for neutrophils. It also activates NK cells and CD4+ and CD8+ T lymphocytes. Additionally, CCL7 has been shown to interact with MMP2. |
