| Formula: |
C14H21N3O · 2HCl |
| Description: |
Y-27632 is a highly potent and selective ATP-competitive inhibitor of Rho-associated kinases (ROCK), exhibiting Ki values of 0.22 µM for ROCK1 and 0.30 µM for ROCK2. In vitro kinase assays reveal that Y-27632 has a 20-fold higher preference for ROCK compared to citron kinase and protein kinase N. Studies indicate that Y-27632 treatment in cells inhibits ROCK-mediated phosphorylation of myosin phosphatase subunit 1 (MYPT1) at Thr853 and Thr696, with a significantly stronger inhibition at Thr853. Additionally, Y-27632 selectively inhibits smooth muscle contraction by preventing Ca2+ sensitization. Further research shows that Y-27632 reduces dissociation-induced apoptosis in cultured human embryonic stem cells over multiple passages and enhances cloning efficiency. |
| Purity: |
> 98% |
| Packing: |
Y-27632 is supplied as a lyophilized powder. |
| Storage: |
Store lyophilized or in solution at -20°C, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. |
| Molecular Weight: |
320.3 g/mol |
| CAS Number: |
129830-38-2 |
| Solubility: |
Soluble in DMSO at 20mg/ml and H2O at 14 mg/mL. |
