Y-27632

2-1-1-green-tea-extract-1

Y-27632

Cat. No.: SPOSGO00049
Y-27632 is a highly potent and selective ATP-competitive inhibitor of Rho-associated kinases (ROCK), exhibiting Ki values of 0.22 µM for ROCK1 and 0.30 µM for ROCK2. In vitro kinase assays reveal that Y-27632 has a 20-fold higher preference for ROCK compared to citron kinase and protein kinase N. Studies indicate that Y-27632 treatment in cells inhibits ROCK-mediated phosphorylation of myosin phosphatase subunit 1 (MYPT1) at Thr853 and Thr696, with a significantly stronger inhibition at Thr853. Additionally, Y-27632 selectively inhibits smooth muscle contraction by preventing Ca2+ sensitization. Further research shows that Y-27632 reduces dissociation-induced apoptosis in cultured human embryonic stem cells over multiple passages and enhances cloning efficiency.
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Description: Y-27632 is a highly potent and selective ATP-competitive inhibitor of Rho-associated kinases (ROCK), exhibiting Ki values of 0.22 µM for ROCK1 and 0.30 µM for ROCK2. In vitro kinase assays reveal that Y-27632 has a 20-fold higher preference for ROCK compared to citron kinase and protein kinase N. Studies indicate that Y-27632 treatment in cells inhibits ROCK-mediated phosphorylation of myosin phosphatase subunit 1 (MYPT1) at Thr853 and Thr696, with a significantly stronger inhibition at Thr853. Additionally, Y-27632 selectively inhibits smooth muscle contraction by preventing Ca2+ sensitization. Further research shows that Y-27632 reduces dissociation-induced apoptosis in cultured human embryonic stem cells over multiple passages and enhances cloning efficiency.
Purity: > 98%
Storage: Store lyophilized or in solution at -20°C, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
CAS No.: 129830-38-2
Molecular Weight: 320.3 g/mol
Formula: C14H21N3O · 2HCl
Solubility: Soluble in DMSO at 20mg/ml and H2O at 14 mg/mL.
Packing: Y-27632 is supplied as a lyophilized powder.

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